Publication details

Aldehyde Oxidase Assay by CE: from off-line, on-line up to immobilized enzyme reactor

Authors

HUANG Shengyun CELÁ Andrea GLATZ Zdeněk VAN SCHEPDAEL Ann ADAMS Erwin

Year of publication 2020
Type Article in Periodical
Magazine / Source JOURNAL OF SEPARATION SCIENCE
MU Faculty or unit

Faculty of Science

Citation
Web https://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=2&SID=E65h1IsqpJJ3rlAVfsY&page=1&doc=1
Doi http://dx.doi.org/10.1002/jssc.202000412
Keywords Enzymes; Assay; Capillary electrophoresis; IMER
Description CE is a modern separation technique characterized by many benefits, which qualify it also for enzyme assays and the study of enzyme kinetics during drug development. Homogenous or heterogenous approaches can be followed for the enzymatic incubation. In this study, an immobilization procedure of aldehyde oxidase on magnetic particles was developed considering their integration with CE. A number of magnetic nano/microparticle types were tested for this purpose, showing that aldehyde oxidase was most active when immobilized on bare silica magnetic nanoparticles.
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