Publication details

In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes

Authors

KASHKAN Ivan TIMOFEYENKO Kseniya ŠLIKOVÁ Eva RŮŽIČKA Kamil

Year of publication 2020
Type Article in Periodical
Magazine / Source Plants
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://www.mdpi.com/2223-7747/9/7/868
Doi http://dx.doi.org/10.3390/plants9070868
Keywords alternative splicing; auxin; cytokinins; brassinosteroids; AHP6; DWF4; PIN7; D-amino acid oxidase; genetic screen; Arabidopsis
Description Rapid progress in plant molecular biology in recent years has uncovered the main players in hormonal pathways and characterized transcriptomic networks associated with hormonal response. However, the role of RNA processing, in particular alternative splicing (AS), remains largely unexplored. Here, using example genes involved in cytokinin signaling, brassinosteroid synthesis and auxin transport, we present a set of reporters devised to visualize their AS events in vivo. These reporters show a differential tissue-specific expression of certain transcripts and reveal that expression of some of the them can be changed by the application of the exogenous hormone. Finally, based on the characterized AS event of the PIN7 auxin efflux carrier, we designed a system that allows a rapid genetic screening for the factors upstream of this AS event. Our innovative toolset can be therefore highly useful for exploring novel regulatory nodes of hormonal pathways and potentially helpful for plant researchers focusing on developmental aspects of AS.
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