Epidemiologická situace čtyř patogenů u koček, jak je to s toxoplasmou u klíšťat?
|Title in English||Epidemiological situation of four feline pathogens, how does toxoplasma infection look like in ticks|
|Year of publication||2020|
|Type||Appeared in Conference without Proceedings|
|MU Faculty or unit|
|Description||An integral part of the spread of pathogens through vectors are their reservoirs in the form of vertebrates, for humans the cat plays a major role in this sense.In the experimental part of the work, the prevalence of a group of 357 cats on four selected pathogenic microorganisms Toxoplasma gondii, Borrelia burgdorferi sensu lato, Coxiella burnetii and Francisella tularensis was investigated. All blood sera came from patients - cats from the Clinic of Diseases of Dogs and Cats, VFU Brno. An ELISA method was used to detect the presence of specific IgM and IgG antibodies in cat sera. The highest seropositivity of cats was shown for the pathogens Toxoplasma gondii and Borrelia burgdorferi sensu lato, followed by Coxiella burnetii, and the fewest cats were positive for antibodies to Francisella tularensis. Furthermore, the work revealed a relatively large percentage of co-infection (in 110 cases) - infections of 2 or more pathogens simultaneously.By monitoring the process of transmissible transmission of pathogens, we also focused on ticks, which we examined by PCR. Ticks were collected at regular intervals throughout the year in Brno-Líšeň, and thus also characterized the area in terms of the risk of pathogen transmission. From the total number of 197 ticks in 68 samples, the positivity for Toxoplasma gondii was determined by rtPCR in 18.75%, the occurrence by PCR was 5%, while the positivity for Bbsl was 13.2%. In conclusion, it can be said that the detected occurrence of all antibodies in the examined group of animals does not exceed the values reported in the literature. There is only sporadic information on the presence of toxoplasma DNA in ticks. We confirmed that the transmissible transmission of toxoplasma between the cat and the tick exists, but it is not clear whether this protozoan is spreading. There should be paid more attention to feline zoonotic diseases, as cats very often share a single home with humans and are thus a potential source of human infection.|