Publication details
Vliv anestezie na rychlost průtoku ve větvích zadní mozkové tepny při transkraniálním dopplerovském vyšetření u potkana
| Title in English | Effect of anesthesia on flow velocity in the branches of the posterior cerebral artery during transcranial Doppler examination in the rat. |
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| Authors | |
| Year of publication | 2023 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | The choice of anesthesia has a major influence on the course and impact of thromboembolic stroke in experiments. Given our efforts to produce and use clinically relevant models, we need information on the effect of anesthetics on cerebral vascular flow. The first step is to describe the effect of anesthesia in healthy intact normotensive animals. In the next steps in hypertensive animals with metabolic syndrome and further in the middle cerebral artery occlusion model in hypertensive animals with metabolic syndrome. The lecture describes the results in a normotensive intact population. The article compares the effect of 1/ general anesthesia induced by the initial inhalation of 2.5% isoflurane, further deepened and guided by the application of a mixture of ketamine (5 mg/kg) + xylazine (35 mg/kg) i.m. (divided into two places and i.p. applied diazepam (2 mg/kg) and for 2/ the effect of general anesthesia induced by the initial inhalation of 2.5% isoflurane, further deepened and guided by ketamine (5 mg/kg) + xylazine (35 mg/kg) i.m. and a mixture of 8% urethane and 0.6% alpha-chloralose in a dose of 8 ml/kg applied i.p. Transcranial doppler is performed using a VEVO 2100 ultrasound tomograph, without violating the integrity of the skin and skull as a non-invasive repeatable registration analogous to clinical practice. The combinations were tested on outbred Wistar males in a wide weight range of 200-450 g. The results and suitability of anesthetic combinations for neurovascular research are discussed in the lecture. |
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