Publication details
Utilizing novel cationic phenylpyridine labeling in glycan profiling by capillary electrophoresis coupled to mass spectrometry
| Authors | |
|---|---|
| Year of publication | 2025 |
| Type | Article in Periodical |
| Magazine / Source | Talanta |
| MU Faculty or unit | |
| Citation | |
| web | https://pdf.sciencedirectassets.com/271360/1-s2.0-S0039914024X00168/1-s2.0-S0039914024017582/main.pdf?X-Amz-Security-Token=IQoJb3JpZ2luX2VjEL3%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJGMEQCIANSGXsRZGPu1HprEwy%2B1AEldjikegAuRXomTdd4JFhQAiBeNQPmukShH |
| Doi | http://dx.doi.org/10.1016/j.talanta.2024.127376 |
| Keywords | Oligosaccharides; Glycans; Labeling; Phenylpyridine; Capillary electrophoresis; Mass spectrometry |
| Description | This work focuses on profiling N-linked glycans by capillary electrophoresis coupled to mass spectrometry using a novel fluorescent and mass spectrometry (MS) active derivatization tag. The label is based on 2-phenylpyridine bearing tertiary amine and hydrazide functionalities. It provides efficient labeling via hydrazone formation chemistry, promising fluorescence properties, and ionization efficiency in the positive ion MS mode. Electrophoretic analysis in a neutral-coated capillary allowed baseline separation of maltooligosaccharides with limits of detection in nanomolar concentrations. The developed labeling method was successfully applied to the analyses of N-linked glycans released from several glycoproteins such as bovine ribonuclease B, human immunoglobulin G, or chicken albumin. |