Publication details
Synthesis and Application of Novel Fluorescent Labeling Tags for Oligosaccharide and N-Linked Glycan Analysis
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| Year of publication | 2023 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
| Citation | |
| Description | Monitoring glycosylation and investigating the influence of glycan structures on their function is an essential area of research, particularly in the diagnosis of disease and the study of mechanisms of pathogenesis.1 Analysis of glycans is still arduous because of their extreme structure complexity and conjugation diversity. But there are also instrumental aspects, such as the sensitivity limits of analyses.2 Therefore, glycan analysis is very often combined with fluorescence detection in addition to MS. In this work, we present the synthesis of novel fluorescent labeling tags for oligosaccharide and N-linked glycan analysis based on the hydrazide derivative of dipyrrometheneboron difluoride (BODIPY) and derivatives of 1,3-diaza-tricyclo[7.3.0.03,7]dodeca-5,7,9,11-tetraene-2,4-dione. Furthermore, we present the application of a newly synthesized hydrazide derivative of BODIPY for oligosaccharide and glycan analysis by high-performance liquid chromatography with fluorescence detection (HPLC/FLD). The labeling method was optimized on maltooligosaccharide standards. The analysis provided the detection limit in the low tens of femtomole, which is at least 30 times more sensitive than the standard approach. In addition, this labeling method was used for N-linked glycan profiling of several glycoproteins by RP-HPLC and HILIC/FLD analysis. |
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