Publication details

Understanding the effect of organic pollutant fluoranthene on pea in vitro using cytokinins, ethylene, ethane and carbon dioxide as indicators

Authors

KUMMEROVÁ Marie VÁŇOVÁ Lucie FIŠEROVÁ Helena KLEMŠ Marek ZEZULKA Štěpán KRULOVÁ Jana

Year of publication 2010
Type Article in Periodical
Magazine / Source Plant Growth Regulation
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1007/s10725-010-9462-0
Field Physiology
Keywords fluoranthene; cytokinin; ethylene; photosynthesis; Pisum sativum L.; in vitro;
Description Environmental contaminants like polycyclic aromatic hydrocarbons can influence many biochemical and physiological processes in plants. The effect of 0.1, 1 and 5 mg l-1 of fluoranthene (FLT) in combination with indole-3-acetic acid (IAA, 0.1 mg l-1) or a combination of IAA and N6-benzyladenine (BA, both 0.1 mg l-1) on the growth and production of ethylene, ethane and CO2 in Pisum sativum L. cultivated for 21 days in vitro was investigated. In 21 days old plants also net photosynthesis rate, content of FLT and cytokinins were evaluated. FLT 5 mg l-1 significantly inhibited the growth of pea after 21 days in both IAA and IAA+BA treatments, increased production of ethylene (by 11% in IAA and 14% in IAA+BA treatments, respectively) and ethane (by 28 and 18%) and decreased production of CO2 (by 23 and 29%). The net photosynthesis rate decreased in response to FLT concentration by up to 51% under saturating irradiation (600–1200 umol m-2 s-1), as found in IAA+BA+FLT 5 mg l-1 treatment. The content of FLT in pea plant shoots well correlated with increasing FLT treatment in both IAA and IAA+BA medium. The content of cytokinins in pea shoots changed in response to FLT treatment. FLT 5 mg l-1 caused a rise in level of trans-zeatin (by 16% in IAA and 9% in IAA+BA treatments, respectively), dihydrozeatin riboside (by 27 and 50%), benzyladenine (by 3 and 80%), benzyladenine riboside (by 44 and 17%) and meta-topolin riboside (by 139 and 214%), no change in isopentenyladenine level and a decrease in metatopolin level (by 33% in IAA and 36% in IAA+BA treatments, respectively). Cultivation of plants in vitro allowed not only to assess their growth, photosynthetic activity and the level of cytokinins, but also to extend the knowledge about the effect of PAHs on production of gaseous stress indicators like ethylene, ethane and CO2. Recorded changes in all studied parameters show, that persistent organic pollutants like PAHs can negatively influence plant growth and development.
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