Publication details

The role of c-Myb in control of migration, invasiveness and death of colon carcinoma cells

Authors

PEKARČÍKOVÁ Lucie KNOPFOVÁ Lucia BURŠÍKOVÁ Jana POTĚŠIL David KONEČNÁ Hana ŠMARDA Jan

Type Article in Proceedings
Conference The Student Scientific Conference on Biotechnology & Biomedicine
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords c-Myb; migration; invasivity; death; colon carcinoma cells
Description c-Myb is a transcription factor that is essential for hematopoiesis and plays important role in formation of colon epithelium. Overexpression of c-Myb has been associated with oncogenic activity and poor prognosis of colon carcinoma patients. Mechanism how c-myb controls the colon tumorigenesis remains unclear. Our results show that overexpressed c-Myb significantly potentiated migration and invasiveness of cells CT26. We analysed secretome of CT26MYBup and control cells by 2-DE followed by LC-MS/MS analysis of differential protein spots. We found protein MIF and galectin-1 as a proteins differentially secreted from CT26MYBup and control cells. c-Myb can regulate tumorigenicity of cells by enhancing of migration and invasive activity, by secretion of MIF and galectin-1 and by imposing resistance of cells to chemoterapeutics.
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