Project information

Analysis of gastrointestinal microbiome in patients with primary immune deficiencies

Project Identification
MUNI/M/1322/2015
Project Period
3/2016 - 12/2018
Investor / Pogramme / Project type
Masaryk University
MU Faculty or unit
Faculty of Medicine
Other MU Faculty/Unit
Faculty of Informatics
Other MU Faculty/Unit
Central European Institute of Technology

In the past decade, gut microbiota has been shown to be associated with a number of human diseases including metabolic syndrome, obesity-related diseases, inflammatory bowel disease, and colorectal cancer. In this project, we plan to study human microbiome in primary antibody immune deficiencies, common variable immunodeficiency (CVID) and selective IgA deficiency (IgAD). In both diseases, IgA production is seriously affected. Since IgA is the dominant mucosal immunoglobulin and plays a central role in maintaining intestinal homeostasis, it is expected that i) the gut microbiome reflects changes in host mucosal immune system. In addition, since there is a significant portion of patients without clinical symptoms or with very mild symptoms, it is likely that ii) the microbiome composition is of importance in these inapparent disease cases. To determine microbiome composition, 16S rRNA gene sequences from the gut microbiome will be examined in all DNA samples (n =50) isolated from patients and healthy controls. In addition, analysis of gut metagenome by whole-metagenome shotgun sequencing and subsequent analysis of gut metabolome will be performed in 6-10 samples previously characterized by the analysis of 16S rRNA gene sequences. This will allow characterization of microbial communities present in the samples and determination of alpha and beta diversity of the samples. In addition, functional characterization of human microbiome will be performed together with determination of the host’s metabolome in a number of selected samples.

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