Publication details

Structure-function Relationships and Engineering of Haloalkane Dehalogenases.

Authors

KOKKONEN Piia Pauliina KOUDELÁKOVÁ Táňa CHALOUPKOVÁ Radka DANIEL Lukáš PROKOP Zbyněk DAMBORSKÝ Jiří

Year of publication 2017
Type Chapter of a book
MU Faculty or unit

Faculty of Science

Citation
Description The structure-function relationships for haloalkane dehalogenases, one of the best characterized enzyme families involved in degradation of halogenated compounds, is described. A substantial amount of mechanistic and structural information is currently available on haloalkane dehalogenases, providing good theoretical framework for their modification by protein engineering. Examples of constructed mutants include variants with modified: (i) activity and specificity, (ii) stability, and (ii) enantioselectivity. Many variants carried mutations in the tunnels connecting the buried active site with surrounding solvent, rather than in the active site itself. Mutagenesis of residues lining the protein tunnels represents attractive and a viable approach of protein engineering.
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