Informace o publikaci

Structures of L-BC virus and its open particle provide insight into Totivirus capsid assembly

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GRYBCHUK Danyil PROCHÁZKOVÁ Michaela FÜZIK Tibor KONOVALOVAS Aleksandras SERVA Saulius YURCHENKO Vyacheslav PLEVKA Pavel

Rok publikování 2022
Druh Článek v odborném periodiku
Časopis / Zdroj Communications Biology
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://www.nature.com/articles/s42003-022-03793-z
Doi http://dx.doi.org/10.1038/s42003-022-03793-z
Klíčová slova Animals; Capsid; Capsid Proteins; Cryoelectron Microscopy; Totivirus; Viruses
Přiložené soubory
Popis L-BC virus persists in the budding yeast Saccharomyces cerevisiae, whereas other viruses from the family Totiviridae infect a diverse group of organisms including protists, fungi, arthropods, and vertebrates. The presence of totiviruses alters the fitness of the host organisms, for example, by maintaining the killer system in yeast or increasing the virulence of Leishmania guyanensis. Despite the importance of totiviruses for their host survival, there is limited information about Totivirus structure and assembly. Here we used cryo-electron microscopy to determine the structure of L-BC virus to a resolution of 2.9 angstrom. The L-BC capsid is organized with icosahedral symmetry, with each asymmetric unit composed of two copies of the capsid protein. Decamers of capsid proteins are stabilized by domain swapping of the C-termini of subunits located around icosahedral fivefold axes. We show that capsids of 9% of particles in a purified L-BC sample were open and lacked one decamer of capsid proteins. The existence of the open particles together with domain swapping within a decamer provides evidence that Totiviridae capsids assemble from the decamers of capsid proteins. Furthermore, the open particles may be assembly intermediates that are prepared for the incorporation of the virus (+) strand RNA. A 2.9 angstrom resolution structure of the L-BC virus provides insight into the contacts between capsid proteins and the mechanism of capsid assembly.
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