Intercellular communication in the development of peritoneal adhesions – focused on the role of extracellular vesicles
- Kód projektu
- Období řešení
- 2/2020 - 12/2020
- Investor / Programový rámec / typ projektu
- Masarykova univerzita
- Fakulta / Pracoviště MU
- Přírodovědecká fakulta
Peritoneal adhesions represent one of the major complications following intra-abdominal surgery leading to symptoms such as abdominal pain, bowel obstruction and infertility. Adhesion formation is primarily caused by activation of mesothelial layer and underlying tissues in peritoneal membrane resulting in transition of mesothelial cells and fibroblasts to pro-fibrotic phenotype. Communication between immune cells, mesothelial cells and fibroblasts is of fundamental importance in this process.
Extracellular vesicles (ectosomes and exosomes) are membrane-derived particles that can reflect their cellular origin and functional state through the bioactive cargoes they carry. We hypothesize that extracellular vesicles represent crucial mediators affecting fibrotic tissue formation in hypoxic stress-related diffuse peritoneal adhesions. To prove this concept, I will isolate and characterize different subtypes of extracellular vesicles in peritoneal fluid, define their origin from maternal cells and determine their functional capacity in adhesion formation.
Understanding the exact role of extracellular vesicles in the development of fibrosis will advance therapeutic methods targeting extracellular vesicle formation to prevent a development of peritoneal adhesions and to protect physiological functions of peritoneal membrane.