Informace o publikaci

Characteristics and distribution of plasmids in a clonally diverse set of methicillin-resistant Staphylococcus aureus strains

Název česky Charakteristiky a distribuce plazmidů u klonálně rozmanitého souboru meticilin-rezistentních kmenů Staphylococcus aureus
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KUNTOVÁ Lucie PANTŮČEK Roman RÁJOVÁ Jana RŮŽIČKOVÁ Vladislava PETRÁŠ Petr MAŠLAŇOVÁ Ivana DOŠKAŘ Jiří

Rok publikování 2012
Druh Článek v odborném periodiku
Časopis / Zdroj Archives of Microbiology
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://link.springer.com/article/10.1007%2Fs00203-012-0797-y
Doi http://dx.doi.org/10.1007/s00203-012-0797-y
Obor Genetika a molekulární biologie
Klíčová slova Methicillin-Resistant Staphylococcus aureus; Plasmids; Genetic diversity; Antibiotic resistance; Molecular typing
Přiložené soubory
Popis The aim of this study was to compare the plasmid contents of methicillin-resistant Staphylococcus aureus (MRSA) strains classified into different clonal clusters (CCs). The isolates were collected from 15 Czech hospitals in 2000–2008. Plasmid DNA was detected in 65 (89%) strains and 33 of them harbored more than one plasmid type. Altogether 24 different types of plasmids were identified, ranging in size from 1.3 to 55 kb. Restriction endonuclease analysis, plasmid elimination, DNA hybridization, and sequencing were used for their further characterization. It has been found that the conjugative, erythromycin resistance and enterotoxin D encoding plasmids are harbored by strains from different CCs. On the other hand, chloramphenicol and tetracycline resistance plasmids, and most of the penicillinase and cryptic plasmids were only detected in certain CCs. Especially the pUSA300-like plasmids were found exclusively in the USA300 clone strains. The high diversity in plasmid content detected in the study strains implies that plasmids play a major role in evolution of MRSA clonal lineages.
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