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Gelatin-templated Nanostructured Gold for Electrochemical Detection of Glucose in Serum

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FARKA Zdeněk JUŘÍK Tomáš PODEŠVA Pavel KOVÁŘ David FORET František SKLÁDAL Petr

Rok publikování 2016
Druh Konferenční abstrakty
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
Popis Nanostructured electrodes based on gelatin-templated gold were developed for sensitive non-enzymatic detection of glucose. The structures of specific saw tooth-like morphology were formed by electrodeposition of gold through the cavities of crosslinked gelatin. The growth of nanostructures was investigated by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The electroactive surface area (ESA) and electrochemical behavior of the prepared electrodes were determined by the cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The roughness factor (expressed as ESA divided by geometrical area) of the gelatin-templated surface was estimated to be 16.7, which represent 15.9 fold enlargement compared to the flat gold electrode. The nanoscopic surface exhibited strong electrocatalytic effect towards glucose oxidation. The detection of glucose was done by direct electrochemical oxidation during CV and amperometry. In case of CV, the sensitivity of 1165 µA·mM-1·cm-2, wide linear range from 32 µM to 10 mM and limit of detection (LOD) of 90 µM were achieved. The amperometry allowed to further improve the LOD to 1.5 µM with very low applied potential of 0 V vs Ag. The detection of glucose in deproteinized human serum samples was possible with negligible interference of oxidizable blood components. The results were verified by a commercial glucometer and standard kit for photometric detection of glucose. Strong correlation between the methods was confirmed; the determination of glucose in serum was performed with a relative standard deviation of 4 %. The developed sensor exhibits a great potential for rapid and sensitive non-enzymatic glucose detection in clinical applications.
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