Informace o publikaci

Cyclopropylamine plasma polymer surfaces for label-free SPR and QCM immunosensing of Salmonella

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MAKHNEVA Ekaterina FARKA Zdeněk SKLÁDAL Petr ZAJÍČKOVÁ Lenka

Rok publikování 2018
Druh Článek v odborném periodiku
Časopis / Zdroj Sensors and Actuators B: Chemical
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://www.sciencedirect.com/science/article/pii/S0925400518314680
Doi http://dx.doi.org/10.1016/j.snb.2018.08.055
Klíčová slova Plasma polymerization; Amine films; Surface plasmon resonance; Quartz crystal microbalance; Immunosensor; Label-free detection
Popis We report on the first successful application of nitrogen-containing functional surfaces prepared by plasma polymerization in surface plasmon resonance (SPR) immunosensing. The plasma polymers (PPs) were deposited from cyclopropylamine (CPA) vapors onto gold surfaces of SPR and quartz crystal microbalance (QCM) sensors. It provided an excellent platform for a stable immobilization of antibodies using glutaraldehyde (GA) activation. The performance of the SPR immunosensor was proven by a model pair of monoclonal antibody (Ab) AL-01 and human serum albumin as well as by the selective detection of bacterial pathogen Salmonella Typhimurium using the immobilized anti-Salmonella Ab. A baseline drift of the measured SPR signal, caused by the long-term reactivity of CPA plasma polymers, was well stabilized by 18 h immersion in phosphate buffered saline prior to the GA activation. The immunosensors can be successfully regenerated several times using 10 mM NaOH. The limit of detection of 10^5 CFU/mL and a wide linear response were achieved for Salmonella. The assay parameters are comparable to the conventional label-free methods. However, the replacement of conventional immobilization matrix with the CPA PPs offers an advantage of excellent adhesion to gold QCM and SPR surfaces achieved by fast and eco-friendly procedure compatible with the vacuum deposition of gold.
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