Informace o publikaci

Online solid-phase extraction liquid chromatography-mass spectrometry of hair cortisol using a surrogate analyte

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KOSTOLANSKÁ Katarína NOVOTNÁ Lucie TÁBORSKÁ Eva PEŠ Ondřej

Druh Článek v odborném periodiku
Časopis / Zdroj Chemical Papers
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://link.springer.com/article/10.1007/s11696-018-0560-1
Doi http://dx.doi.org/10.1007/s11696-018-0560-1
Klíčová slova Endogenous cortisol; Long-term hair concentration; Surrogate analyte LC–MS; Online SPE
Popis Online SPE LC–MS with 13C3-cortisol as a surrogate analyte was developed, validated and applied for obtaining hair cortisol levels of 114 healthy volunteers. The first 3 cm from a posterior vertex position were analyzed as three individual segments resulting in 310 hair cortisol levels, which were used, after log transformation, to predict a range of values observed in future. The median value of hair cortisol was 4.76 pg/mg. The employed method allowed simple processing, high throughput and may be readily expanded to analyze additional steroid compounds in hair.
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