Informace o publikaci

Epigenetic pattern of plant telomeres with non-canonical sequence

Název česky Epigenetický vzor telomer u rostlin s nekanonickou sekvencí


Rok publikování 2019
Druh Další prezentace na konferencích
Fakulta / Pracoviště MU

Středoevropský technologický institut

Popis Telomeres are nucleoprotein structures at the ends of linear eukaryotic chromosomes. They are crucial for the maintenance of genome integrity protecting the genetic information inside the chromosomes from the lost due to the incomplete DNA replication, and distinguishing natural chromosome ends from DNA breaks. Telomeres consist of tandemly repeated short units, sequence of which is relatively conserved, e.g., TTAGGG in mammals or TTTAGGG in most plants. Recently, plants with non-canonical telomeres were described, including Genlisea hispidula (TTCAGG/TTTCAGG), Cestrum elegans (TTTTTTAGGG), plants from several families in the Asparagales order (TTAGGG), and Allium species (CTCGGTTATGGG). We analyzed epigenetic properties of telomere histones in plants with non-canonical telomeres (C. elegans, G. hispidula, A. cepa, Narcissus pseudonarcissus) and with canonical telomeres (A. thaliana, N. tabacum, N. benthamiana, Genlisea nigrocaulis). Next, we analyzed telomere chromatin captured from N. benthamiana leaves transiently transformed by telomere CRISPR-dCas9-eGFP, and from A. thaliana stably transformed with TALE_telo C-3xGFP. Chromatin was immunoprecipitated with antibodies against specific histone marks and DNA was hybridized with telomere probe. Despite of differences in telomere lengths and sequences, tested plant species exhibited two kinds of epigenetic patterns of telomere histones. i) Arabidopsis – like pattern (A. thaliana, G. hispidula, G. nigrocaulis, A. cepa, N. pseudonarcissus), with telomere histones decorated predominantly by H3K9me2. ii) tobacco – like pattern (N. tabacum, N. benthamiana, C. elegans), with telomere histones decorated predominantly by H3K27me3. Our data suggests that epigenetic modifications of plant telomere histones are directly related neither to the length of telomeres, nor to their particular sequence.
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