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SOLVING PHASE PROBLEM USING A SE-MET DERIVATIVE OF THE FLAVOENZYME NAD(P)H:ACCEPTOR OXIDOREDUCTASE (FERB)

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KLUMPLER Tomáš MAREK Jaromír SEDLÁČEK Vojtěch KUČERA Igor

Rok publikování 2010
Druh Článek ve sborníku
Konference Materials Structure, vol. 17, no. 1a (2010)
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Obor Biochemie
Klíčová slova phase problem; flavoenzyme; FerB; reductase; crystallography
Popis The flavin adenine dinucleotide-dependent enzyme FerB from Paracoccus denitrificans reduces a range of sub strates, including chromate, ferric complexes, benzoquinones and naphtoquinones. The reduced form of nicotinamide adenine dinucleotide serves as a source of electrons. Recombinant unmodified and selenometionine-substituted (Se-Met) FerB derivatives were crystallized, diffraction data for both forms were collected and the phase problem for Se-Met FerB dimer was solved by three-wavelength multiple anomalous dispersion, followed by the combination of molecular replacement and single-wavelength anomalous diffraction phasing. A molecular replacement solution of unmodified FerB tetramer was obtained using Se-Met structure as a search model.
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