Informace o projektu

Capillary electrophoresis as a member of the metabolomic analytical toolbox (CE metabolomics)

Kód projektu
GAP206/11/0009
Období řešení
1/2011 - 12/2015
Investor/Program
Grantová agentura ČR
Programový rámec / typ projektu
Standardní projekty
Fakulta/Pracoviště MU
Přírodovědecká fakulta
Další fakulta/pracoviště MU
Lékařská fakulta

The generic methods for metabolomic studies based on application of different CE modes - capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MEKC), microemulsion electrokinetic capillary chromatography (MEEKC), nonaqueous capillary electrophoresis (NACE) for corresponding metabolite classes will be developed. The relevant on-line preconcentration strategies will be integrated in given methods and possibility of using with MS detection will be tested. They can be used for metabolite fingerprinting and footprinting, metabolite profiling as well as target analysis. The complete procedure for the target metabolome analysis of microbial samples thus will be available starting from the rapid metabolism quenching and the quantitative extraction of selected metabolites up to their analysis by different CE modes. Besides the new method for the embryo viability testing based on non-invasive metabolite footprinting and profiling will be available as well.

Publikace

2016

Determination of Caffeine and its Metabolites in Saliva and Urine as a Measure of CYP1A2 Metabolic Activity

TURJAP Miroslav — ZENDULKA OndřejGLATZ ZdeněkBREJCHA StanislavMÁDR AlešJUŘICA Jan

Chapter 11 Bioanalytical methods - Metabolomics

GLATZ Zdeněk

MEKC LIF method for analysis of amino acids after on-capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization

CELÁ AndreaMÁDR Aleš — DĚDOVÁ Tereza — PELCOVÁ Marta — JEŠETA MichalŽÁKOVÁ JanaCRHA IgorGLATZ Zdeněk

Various instrumental approaches for determination of organic acids in wines

ŽERAVÍK Jiří — FOHLEROVÁ Zdenka — MILOVANOVIĆ MiodragKUBESA Ondřej — ZEISBERGEROVA Marta — LACINA Karel — PETROVIC Alexandr — GLATZ ZdeněkSKLÁDAL Petr

2015

Analysis of amino acids based on capillary electrophoresis with LIF detection using on-capillary derivatization

CELÁ AndreaMÁDR Aleš — DĚDOVÁ Tereza — PELCOVÁ Marta — GLATZ Zdeněk

Determination of biochemically important flavins using capillary electrophoresis with laser induced fluorescence detection

CELÁ AndreaMÁDR AlešGLATZ Zdeněk

Determination of pyruvate and lactate as potential biomarkers of embryo viability in assisted reproduction by capillary electrophoresis with contactless conductivity detection

MÁDR AlešCELÁ Andrea — KLEJDUS Bořivoj — PELCOVÁ Marta — CRHA IgorŽÁKOVÁ JanaGLATZ Zdeněk

Development of capillary electrophoresis based method for analysis of metabolic turnover of energetically important compounds as a tool for human embryo quality assessment

MÁDR Aleš — PELCOVÁ Marta — ŽÁKOVÁ JanaCRHA IgorGLATZ Zdeněk

Method development for baseline separation of cyanobenz[f]isoindoles of proteinogenic amino acids by CE-LIF

MÁDR AlešCELÁ Andrea — DĚDOVÁ Tereza — PELCOVÁ Marta — ŽÁKOVÁ JanaCRHA IgorGLATZ Zdeněk

Využití kapilární elektroforézy pro stanovení metabolické aktivity embrya

MÁDR AlešCELÁ AndreaŽÁKOVÁ JanaCRHA IgorGLATZ Zdeněk

Předchozí 1 2 3 4 5 Další

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info