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HPLC-MS Identification of Metabolites of Selected Benzophenanthridine Alkaloids by Rat Liver Microsomes

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MIDLIK Adam ŠEBRLOVÁ Kristýna KUŠNYEROVÁ Ivana DOVRTĚLOVÁ Gabriela NOSKOVÁ Kristýna JUŘICA Jan TÁBORSKÁ Eva PEŠ Ondřej

Rok publikování 2014
Druh Článek ve sborníku
Konference Book of Abstracts European Symposium on Atomic Spectrometry ESAS 2014 & 15th Czech - Slovak Spectroscopic Conference
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Obor Biochemie
Klíčová slova LC MS; benzophenanthridine alkaloids; metabolites; microsomes
Popis Quaternary benzophenanthridine alkaloids (QBAs) are a group of isoquinoline derivatives produced by plants of the Papaveraceae, Fumariaceae and Rutaceae family. Presently they are studied intensively because of their biological activity. Anti-inflammatory, anti-microbial, anti-fungal, antiparasitic, as well as pro-apoptotic and anti-tumor effects of QBAs have been reported. Determination of toxicity of these potential drugs is based on metabolic studies of major, commercially available sanguinarine (SA) and chelerythrine (CHE). However, metabolic fate and pharmacological activity of minor QBAs, such as chelilutine (CL), chelirubine (CR), sanguilutine (SL), sanguirubine (SR) and macarpine (MA), might differ considerably, despite their structural similarity. Rat liver microsomes (RLMs), subcellular particles containing metabolizing enzymes, such as a cytochrome P450 family, provide a simple metabolic model for in vitro investigation before carrying out in vivo tests.
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