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Elicititns Oligomerization: What Could Clarify in Elicitins Actvity

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SOLANSKÝ Martin SKLENÁŘ Matěj PĚTROŠ Vojtěch LEBORNE-CASTEL Nathalie LOCHMAN Jan

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Fakulta / Pracoviště MU

Přírodovědecká fakulta

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Popis A well described and accessible model of plant-pathogen interaction is represented by elicitins and Solanaceae plants. Elicitins, a family of small proteins secreted by Phytophthora and Pythium spp., are the most well-known microbe-associated molecular patterns of oomycetes (MAMPs). Recently, it has been described that one of the factors affecting elicitins activity in plants could be represented by their ability to form dimer/oligomer under normal conditions. To clear up in details the role of dimer within elicitins biological activity we prepared covalently crosslinked dimer of highly necrotising elicitin ?-cryptogein (?-CRY) and its variants D21N and K13V carrying mutations in the region likely involved in dimerization. By analytical ultracentrifugation we proved different KD of homodimer complexes formation in both mutants and wt ?-CRY. Further, we found a pretty relation between proteins KD of dimer formation and response of tobacco plants and cell suspension to their treatment. Noticeable, we found that ?-CRY dimer is affected in interaction with receptor-like protein ELR from S. microdontum and in triggering of ROS production in tobacco cells but not in activation of WIPK/SIPK kinases leading to defense genes expression. Consequently, we studied on the model of BY2 cells and GP3 cells, impaired in NADPH oxidase, the exact role of ROS in early and late phase of plant-elicitin interaction.
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