Informace o publikaci

Generation of human iPSCs from fetal prostate fibroblasts HPrF

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KAHOUNOVA Zuzana SLABAKOVA Eva BINO Lucia REMSIK Jan FEDR Radek BOUCHAL Jan VRTEL Radek JUREČKOVÁ Lucie POROKH Volodymyr PARALOVA Darja HAMPL Aleš SOUCEK Karel

Rok publikování 2019
Druh Článek v odborném periodiku
Časopis / Zdroj Stem cell research
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://www.sciencedirect.com/science/article/pii/S1873506119300352
Doi http://dx.doi.org/10.1016/j.scr.2019.101405
Klíčová slova iPSCs; fetal prostate fibroblasts
Popis Human induced pluripotent stem cell line was generated from commercially available primary human prostate fibroblasts HPrF derived from a fetus, aged 18-24 weeks of gestation. The fibroblast cell line was reprogrammed with Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using CytoTune (TM)-iPS 2.0 Sendai Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting the expression of factors of pluripotency on a single-cell level, and in vivo using teratoma formation assay. This iPS cell line will be a useful tool for studying both normal prostate development and prostate cancer disease.
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