Informace o publikaci

Modeling of the N-Acetylglucosaminyltransferase V (GnT-V)


JANOŠ Pavel KOZMON Stanislav TVAROŠKA Igor KOČA Jaroslav

Rok publikování 2014
Druh Konferenční abstrakty
Fakulta / Pracoviště MU

Středoevropský technologický institut

Popis Glycosyltransferases are group of enzymes, that catalyze the formation of glycosidic linkages by transferring sugar moiety from activated donor substrates to a variety of different acceptor substrates. As such they are responsible for synthesis of complex glycan structures, that have been shown to play crucial roles in cell–cell, cell–matrix and cell–pathogen interactions and thus impact huge range of biological processes. The subject of this study is N-Acetylglucosaminyltransferase V (GnT-V), a glycosyltranferase involved in biosynthesis of asparagine-linked oligosaccharides. Specifically it catalyzes the transfer of GlcNAc moiety from UDP–GlcNAc to the 6 position of the ?-1-6 linked Man residue to the core N-glycans to form branched tri- and tetra-antenna-like oligosaccharides. GnT-V has been linked to cancer growth and metastasis, which makes it an interesting target for drug development. However the lack of structural information on this enzyme is holding these efforts back. In our work, we attempt to devise structural model of GnT-V by means of homology modeling with aims to use it to guide future experiments, to elucidate the catalytic mechanism and to identify amino acids crucial for its function.

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