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Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry

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ŠEDO Ondrej KOŘÁN Michal JAKEŠOVÁ Michaela MIKULÍKOVÁ Renata BOHÁČ Michal ZDRÁHAL Zbyněk

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj Food Chemistry
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://www.sciencedirect.com/science/article/pii/S0308814616304095
Doi http://dx.doi.org/10.1016/j.foodchem.2016.03.056
Obor Analytická chemie, separace
Klíčová slova Barley; Malting; MALDI-TOF MS; Proteins
Popis A method for discriminating malting barley varieties based on direct matrix-assisted laser desorption-ionisation -time-of-flight mass spectrometry (MALDI-TOF MS) fingerprinting of proteins was developed. Signals corresponding to hordeins were obtained by simple mixing of powdered barley grain with a MALDI matrix solution containing 12.5 mg mL(-1) of ferulic acid in an acetonitrile: water: formic acid 50:33:17 v/v/v mixture. Compared to previous attempts at MALDI-TOF mass spectrometric analysis of barley proteins, the extraction and fractionation steps were practically omitted, resulting in a significant reduction in analytical time and costs. The discriminatory power was examined on twenty malting barley varieties and the practicability of the method was tested on sixty barley samples acquired from Pilsner Urquell Brewery. The method is proposed as a rapid tool for variety assignment and purity determination of malting barley that may replace gel electrophoresis currently used for this purpose.
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