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Polymer pencil graphite electrode as a promising sensor for guanine and its analogues

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TRNKOVÁ Libuše TŘÍSKOVÁ Iveta BOSÁKOVÁ Markéta

Druh Článek ve sborníku
Konference International School "Electrochemical and acoustic methods in bioanalysis"
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Obor Fyzikální chemie a teoretická chemie
Klíčová slova polymer pencil graphite elektrode; guanine; guanosine; GMP; SEM; XPS; Raman spectroscopy; linear sweep voltammetry; HOMO energy; quantum chemical calculations; Spartan software
Popis The oxidative electrochemistry of guanine (G - one of the two purine bases in nucleic acids) and its derivatives including the nucleoside and nucleotide monophosphate appears to be of particular interest to compare with biological pathways [1-3]. Different electrodes are useful for the study of oxidation processes. Among diverse electrode materials, carbon is often favored as the key component for construction of electrochemical sensors and biosensors [4-6]. One of carbon-based electrodes is a pencil lead which is easily available at a very low cost [4-6]. Previously, we characterized surfaces of “polymer” branded pencil leads by modern non-electrochemical methods such as scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS) [7]. Here we present that electrochemical techniques in connection with polymer pencil graphite electrodes (pPeGE) are fruitful for the analysis of guanine and its derivatives such as guanosine (Guo), guanosine monophosphate (GMP) and their deoxy-derivatives. The investigation was aimed at oxidation responses of G, Guo and GMP in dependence on pH (phosphate-acetate buffer), accounting for structural increments (ribofuranosyl-, deoxyribofuranosyl and phosphate group). It was found that anodic peak potentials increase in the order G<
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