Heterochromatinization associated with cell differentiation as a model to study DNA double strandbreak induction and repair in the context of higher-order chromatin structure

• Autoři: FALK Martin; LUKÁŠOVÁ Emilie; ŠTEFANČÍKOVÁ Lenka; BARANOVÁ E; FALKOVÁ I; JEŽKOVÁ L; DAVÍDKOVÁ M Bačíková A; VACHELOVÁ J; MICHAELIDESOVÁ A; KOZUBEK Stanislav
• Rok publikování: 2014
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Applied Radiation and Isotopes
• Doi: http://dx.doi.org/10.1016/j.apradiso.2013.01.029
• Popis: Cell differentiation is associated with extensive gene silencing, heterochromatinization and potentially decreasing need for repairing DNA double-strand breaks (DSBs). Differentiation stages of blood cells thus represent an excellent model to study DSB induction, repair and misrepair in the context of changing higher-order chromatin structure. We show that immature granulocytes form ?H2AX and 53BP1 foci, contrary to the mature cells; however, these foci colocalize only rarely and DSB repair is inefficient. Moreover, specific chromatin structure of granulocytes probably influences DSB induction.