Informace o publikaci

Oxidatively-mediated in silico epimerization of a highly amyloidogenic segment in the human calcitonin hormone (hCT(15-19))

Autoři	HAMID A.K.M. SALVATORE J.C. WANG K. MURAHARI P. GULJAS A. RAGYANSZKI A. OWEN Michael Christopher JOJART B. SZORI M. CSIZMADIA I.G. VISKOLCZ B. FISER B.
Rok publikování	2019
Druh	Článek v odborném periodiku
Časopis / Zdroj	COMPUTATIONAL BIOLOGY AND CHEMISTRY
Fakulta / Pracoviště MU	Středoevropský technologický institut
Citace
www	https://www.sciencedirect.com/science/article/pii/S1476927119300222?via%3Dihub
Doi	http://dx.doi.org/10.1016/j.compbiolchem.2019.04.005
Klíčová slova	DFNKF; Amyloidosis; Aggregation; Oxidative-stress; Molecular ageing; Peptide
Popis	In order to study the effects of peptide exposure to oxidative attack, we chose a model reaction in which the hydroxyl radical discretely abstracts a hydrogen atom from the alpha-carbon of each residue of a highly amyloidogenic region in the human calcitonin hormone, hCT(15-19). Based on a combined Molecular Mechanics / Quantum Mechanics approach, the extended and folded L- and D-configuration and radical intermediate hCT(15-19) peptides were optimized to obtain their compactness, secondary structure and relative thermodynamic data. The results suggest that the epimerization of residues is generally an exergonic process that can explain the cumulative nature of molecular aging. Moreover, the configurational inversion induced conformational changes can cause protein dysfunction. The epimerization of the central residue to the D-configuration induced a hairpin structure in hCT(15-19) concomitant with a possible oligomerization of human calcitonin into A beta(1-42)-like amyloid fibrils present in patients suffering from Alzheimer's disease.