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Impact of viral infection on the immune defense in hybridizing fish

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Rok publikování 2021
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Popis Transcriptome analysis is considered a powerful tool for unveiling the distribution and magnitude of genetic incompatibilities between hybridizing taxa. Such analysis has been applied to reveal that gene misexpression may underlie reproductive isolation mechanisms and immune mechanisms in interspecific hybrids. The aim of this study was to analyze differential immune expression across experimental lines within the hybridizing system of the phylogenetically closely related cyprinid species – Cyprinus carpio and Carassius gibelio exposed to viral infection (spring viraemia of carp virus, SVCV). Total of nine lines (pure C. carpio, pure C. gibelio, F1 C. carpio x C. gibelio, F1 C. gibelio x C. carpio, 2 lines of maternal backcrosses, 2 lines of paternal backcrosses, and F2 hybrids) were prepared by artificial breeding. Fish specimens were infected by the same dose of SVCV. To evaluate a potential disruption of the immune system, 33 KEGG pathways (including e.g. B cell receptor signalling pathway (ko04662) or antigen processing and presentation (ko04612)) were analysed. Additionally, transcriptomic analysis of spleen revealed numerous genes differentially expressed in infected and control (non-infected) groups, specifically the expression of GIG2 and suPAR in pure C. carpio and F2 hybrids was higher in infected fish with comparison to control fish. ISG15 has shown higher expression levels through infected fish of all lines with comparison to the control fish of all lines. SDF1 gene expression was downregulated in infected fish of all lines when compared to the control fish, which may lead us to better understanding of the possible role in host-parasite interactions.
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