Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin

• Autoři: HRUŠKOVÁ Helena; OLSEN Christine; ŘEMÍNEK Roman; WANG Chencheng; AIZENSHTADT Aleksandra; KRAUSS Stefan; SCHOLZ Hanne; ROBERG-LARSEN Hanne; FORET František; WILSON Steven Ray
• Rok publikování: 2024
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Journal of Chromatography A
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• www: https://www.sciencedirect.com/science/article/pii/S0021967324000426
• Doi: http://dx.doi.org/10.1016/j.chroma.2024.464669
• Klíčová slova: Liquid chromatography-mass spectrometry; Insulin; Preparative gel electrophoresis; Stem cell-derived islet organoids
• Popis: Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal-free studies of development, disease, and drug discovery. The cell medium of organoid models of Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed by liquid chromatography-mass spectrometry (LC-MS). However, organoid medium complexity is a major challenge, as matrix interferences can reduce sensitivity and selectivity, even with optimized LC-MS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the cell medium background signal, allowing for reduced interferences affecting LC-MS analysis of human insulin.