DNA and Chromatin Structure of Telomere-Subtelomere Junctions in Silene latifolia
|Článek v odborném periodiku
|Časopis / Zdroj
|J.Biomol. Struct. Dyn.
|Fakulta / Pracoviště MU
|Genetika a molekulární biologie
|Telomere; Subtelomere; Chromatin; Silene latifolia
|Genomic variants of telomere-subtelomere junctions in Silene latifolia chromosomes were cloned and sequenced. In addition to the previously isolated subtelomeric sequence X43.1 (Buzek et al., 1997), several novel telomere-associated sequences have been characterised, including high-copy tandem repeat 15Ssp and low-copy sequence BstB19. Their subtelomeric location has been confirmed by pulsed-field gel electrophoresis and fluorescent in situ hybridisation. Analysis of chromatin structures (nucleosome phasing, nucleosome positioning) at these sequences revealed their specific features