Bioluminiscenční, elektroforetická a biochemická charakteristika nového českého izolátu z rodu Photorhabdus
|Článek ve sborníku
|Sborník abstraktů Tomáškovy dny 2003
|Fakulta / Pracoviště MU
|We isolated symbiotic bacteria from entomopathogenic nematode Heterorhabditis bacteriophora H221 (Pouzdřany). Our isolate CCM 7002 shows clear luminescence and this is characteristic for genus Photorhabdus, the only known terrestrial luminescent bacteria. Thus our isolate was compared with type cultures of Photorhabdus species and subspecies (P. luminescens ssp. luminescens, P. luminescens ssp. laumondii, P. luminescens ssp. akhurstii, P. asymbiotica and P. temperata). All Photorhabdus strains have been shown luminescence (bioluminescence). Luminescence was measured on luminometer (BioOrbit 1251) at 25 and 37 C for two hours. Luminescent signal is rising at 25 C and at the temperature of 37 C is falling or constant. Each tested strain has a typical luminescent signal. The highest value shows always P. temperata. To compare the protein spectrum of studied bacteria species, bacterial proteins were analysed by acrylamide gradient gel electrophoresis (SDS-PAGGE). We found more than 40 protein fractions in the range of 6.5 200 kDa. We demonstrated significant differences between the species and subspecies of genus Photorhabdus. Phenotypic characteristics were based on identification kits (API 20 E, API 20 NE, ENTEROtest 24) supplemented with additional conventional tests. Identification of the CCM 7002 to the species level remains dubious due to low biochemical activity. Mostly related species to CCM 7002 on the basis of phenotyping is P. luminescens species.