Informace o publikaci
An immunohistochemical staining for p-38 as a marker for satellite cells mediating indirect reactions of the dorsal root ganglia to nerve injury
| Název česky | Imunohistochemická lokalizace p38 jako markru sat.buněk zprostředkujících nepřímé reakce spinálních ganglií na poškození nervu |
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| Autoři | |
| Rok publikování | 2007 |
| Druh | Další prezentace na konferencích |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Poškození nervu indukuje neuropathickou bolest a activuje p38 mitogen-aktivovanou protein kinázu (p38) v určité populaci neuronů spinálních ganglií a v mikroglii míchy. Sledovali jsem imunohistochemickou lokalizaci aktivovaného p38 proteinu v v C7-C8 a L4-L5 spinálních gangliích intaktních a operovaných potkanů pro unilaterální ligaturu L4-L5 spinálního nervu SNL) a sedacího nervu (ScNL) a po transekci sedacího nervu(ScNT). In contrast to published results an enhanced p38-IF was found not only in DRG neurons but also in the satellite cells (SC), particularly after ScNL and ScNT for 1, 2, and 4 weeks. An increased p38-IF was observed in the SC of contralateral L4-5 DRG and those of cervical segments in comparison to very low p38-IF in the cells of ipsilateral L4-5 DRG. In contrast, small and medium-sized neurons displayed increased p38-IF only in the ipsilateral L4-5 DRG. A week SNL induced elevated p38-IF in the cervical DRG neurons, but reduced p38-IF in the ipsi- and contralateral L4-5 DRG. The DRG neurons related to SNL displayed an increased p38-IF until 2 weeks. An increased p38-IF in the SC was observed in DRG not associated with damaged nerve. Our results suggested a nerve damage signaling that spreads in the nervous system to induce expression of the critical p38 mitogen-activated protein kinase. In addition, our results indicate that the signaling in the DRG not associated with injured nerve may be mediated by satellite glial cells. |
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