Informace o publikaci
The influence of Congo red on the cell wall and 1,3-ß-D-glucan microfibril biogenesis in Saccharomyces cerevisiae.
| Autoři | |
|---|---|
| Rok publikování | 1992 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Arch. Microbiol. |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://www.springerlink.com/content/n2n66763n7213404/ |
| Obor | Morfologické obory a cytologie |
| Klíčová slova | : SACCHAROMYCES-CEREVISIAE; YEAST CELLS; YEAST PROTOPLASTS; CELL WALL; CONGO RED; (1-]3)-BETA-D-GLUCAN MICROFIBRILS; CYTOKINESIS; REVERSION OF WALLED PROTOPLASTS TO CELLS |
| Popis | Congo red caused formation of chains of connected cells with aberrant wall structure at septum region. The wall-less protoplasts in solid medium with the dye exhibited an abnormal increase in volume, aberrant regeneration of walls and inability to revert to cells. In liquid medium, the protoplasts synthesized glucan nets composed mainly of thin fibrils orientated at random, whereas normally, in the absence of dye, the nets consist of rather thick fibrils, 10 to 20 nm in width, assembled into broad ribbons. These fibrils are known to consist of triple 6/1 helical strands of -1,3-beta-D-glucan aggregated laterally in crystalline packing. The thin fibrils (4 to 8 nm wide) containing only a few triple helical strands (c. 1.6 nm wide)are supposed to be prevented from further aggregation and crystallization by complexing with Congo red on their surfaces. Some loose triple 6/1 helical strands present the first native 1,3-beta-D-glucan elementary fibrils depicted by electron microscopy. |