Structural rearrangement of the actin cytoskeleton in regenerating protoplasts of budding yeast.

• Autoři: GABRIEL Miroslav; KOPECKÁ Marie; SVOBODA Augustin
• Rok publikování: 1992
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: J. Gen. Microbiol.
• Fakulta / Pracoviště MU: Lékařská fakulta

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• Obor: Ostatní lékařské obory
• Klíčová slova: SACCHAROMYCES-CEREVISIAE; SCHIZOSACCHAROMYCES-POMBE; CELL-CYCLE; GENE; DISRUPTION; TUBULIN; PROTEIN; MUTANTS
• Popis: In Saccharomyces cerevisiae actin cytoskeleton is present as actin dots in the bud and around the septum and as actin cables - loose bundles along the longitudinal cell axis. However, the apparently asymmetric pattern of actin no longer persisted after protoplasting, when the cables disappeared and dots became evenly distributed. This pattern was maintained during regeneration of a new wall all over the protoplast surface, thus providing evidence of a relationship between the new wall formation and the presence of a regular arrangement of actin dots. The completed cell wall allowed the protoplast to bud and produce a normal daughter cell. However, before the walled protoplast began to bud, actin dots accumulated at the site of bud emergence and actin cables appeared, extending to the cytoplasm. Later, actin dots accumulated in the growing bud, forming a ring in the neck, and actin cables passed to the bud. Completion of the protoplast-to-cell reversion was preceded by restoration of actin cytoskeleton.