Informace o publikaci
Outdoor environment as a source of Listeria monocytogenes in food chain
| Název česky | Vnější prostředí jako zdroj Listeria monocytogenes v potravinovém řetězci |
|---|---|
| Autoři | |
| Rok publikování | 2012 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Czech Journal of Food Sciences |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Potravinářství |
| Klíčová slova | virulence; listeria; antimicrobial resistance; restriction fragment length polymorphism; pulsed-field gel electrophoresis (PFGE) |
| Popis | The aims of this study were to monitor the presence of Listeria monocytogenes in environmental sources and to evaluate the phenotypic and molecular characteristics of the isolates recovered. L. monocytogenes was isolated in 12 (11.2%) of the 107 samples from the wild, farm environment, and vegetation. Most isolates (83.3%) were of serotype 1/2a and the remainder (2) were of serotype 4b. All 12 isolates were susceptible to the whole range of antimicrobials tested. These12 strains were carriers of the virulence genes prfA, hlyA, actA, plcA, plcB, inlA, inlB, inlC, and inlJ. The detection of the inlA gene in 4 (33.3%) of 12 strains using the PCR-RFLP suggests the potential of some of these strains to penetrate into epithelial cells of the intestinal barrier. Macrorestriction analysis also confirmed clonal identity of some environmental isolates with food and human isolates. These results indicate that the external environment is a source of potentially pathogenic strains of L. monocytogenes. |