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Mammalian DIS3L2 exoribonuclease targets the uridylated precursors of let-7 miRNAs

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USTIANENKO Dmytro HROŠŠOVÁ Dominika POTĚŠIL David CHALUPNÍKOVÁ Kateřina HRAZDILOVÁ Kristýna PACHERNÍK Jiří CETKOVSKÁ Kateřina ULDRIJAN Stjepan ZDRÁHAL Zbyněk VAŇÁČOVÁ Štěpánka

Druh Článek v odborném periodiku
Časopis / Zdroj RNA
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://rnajournal.cshlp.org/content/early/2013/10/18/rna.040055.113.abstract
Doi http://dx.doi.org/10.1261/rna.040055.113
Obor Genetika a molekulární biologie
Klíčová slova DIS3L2; RNA degradation; RNA uridylation; let-7 miRNA
Popis The mechanisms of gene expression regulation by miRNAs have been extensively studied. However, the regulation of miRNA function and decay has long remained enigmatic. Only recently, 3'uridylation via LIN28A-TUT4/7 has been recognized as an essential component controlling the biogenesis of let-7 miRNAs in stem cells. Although uridylation has been generally implicated in miRNA degradation, the nuclease responsible has remained unknown. Here, we identify the Perlman syndromeassociated protein DIS3L2 as an oligo(U)-binding and processing exoribonuclease that specifically targets uridylated pre-let-7 in vivo. This study establishes DIS3L2 as the missing component of the LIN28-TUT4/7-DIS3L2 pathway required for the repression of let-7 in pluripotent cells.
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