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Structure and function of Photorhabdus asymbiotica lectins: Studies of potential virulence factors from emerging human pathogen

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POKORNÝ Daniel JANČAŘÍKOVÁ Gita KOMÁREK Jan WIMMEROVÁ Michaela

Rok publikování 2016
Druh Konferenční abstrakty
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Popis Lectins are ubiquitous carbohydrate-binding proteins found in bacteria, plants, animals, fungi and viruses where they play a key role in various processes. Our research is focused on lectins from Photorhabdus genus - gram-negative bioluminescent bacteria. Photorhabdus genus contains three species all of which are insect pathogens: P. luminescens, P. temperata and P. asymbiotica. Unlike other species in this genus, P. asymbiotica is also able to infect humans. In the P. asymbiotica genome, three genes of putative lectins named PHL, PHL2 and PHL3 were found. Recombinant lectins were studied using broad range of biophysical methods. For affinity measurements we employed isothermal titration calorimetry, micro-scale thermophoresis and surface plasmon resonance. The oligomeric state of the proteins was determined by analytical ultracentrifugation. We have optimized crystallization conditions using high throughput screening, and diffracting-quality crystals were used for structure determination. Structure-supported functional studies help us to understand molecular details of lectin function and their contribution to pathogenesis. Understanding the lectins role in pathogenic processes may be exploited for designing new therapeutic strategies.
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