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A Comprehensive In Vitro Comparison of Preparation Techniques for Fat Grafting

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STREIT Libor JAROŠ Josef SEDLÁKOVÁ Veronika SEDLÁČKOVÁ Miroslava DRAŽAN Luboš SVOBODA Michal POSPÍŠIL Jakub VÝŠKA Tomáš VESELÝ Jiří HAMPL Aleš

Rok publikování 2017
Druh Článek v odborném periodiku
Časopis / Zdroj PLASTIC AND RECONSTRUCTIVE SURGERY
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1097/PRS.0000000000003124
Obor Chirurgie včetně transplantologie
Klíčová slova reconstructive surgery
Popis Background: Lipomodeling is a technique that uses the patient's own fat for tissue regeneration and augmentation. The extent of regenerative effect is reported to be determined by the numbers of adipose-derived stem cells and the viability of cells in processed adipose tissue which, together with other factors, influence the degree of graft retention. This study addresses whether differences exist in properties of fat graft obtained by three commonly used techniques. Methods: Adipose tissue harvested from the hypogastric regions of 14 patients was processed by decantation, centrifugation, and membrane-based tissue filtration. The morphology of each preparation was assessed by electron microscopy and overall cell viability was assessed by live/dead assay. The number of adiposederived stem cells was determined and their stem cell character was assessed by the presence of cell surface molecules (i.e., CD105, CD90, CD31, and CD45) and by their capacity to differentiate into adipogenic and osteogenic lineages. Results: First, morphologies of processed fat samples obtained by individual procedures differed, but no preparation caused obvious damage to cellular or acellular components. Second, although the highest numbers of adiposederived stem cells were contained in the upper fraction of centrifuged lipoaspirates, the difference between preparations was marginal. Third, the maximal concentration of adipose fraction (removal of watery component) of lipoaspirate was achieved by membrane-based tissue filtration. Finally, no significant differences in overall viability were detected. Conclusions: Properties of processed lipoaspirate were influenced by the preparation procedure. However, the differences were not dramatic; both centrifugation and membrane-based filtration are methods of choice whose selection depends on other criteria (e.g., practicality) for individual surgical settings.
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