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Microbe-focused glycan array screening platform

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GEISSNER Andreas REINHARDT Anika RADEMACHER Christoph JOHANNSSEN Timo MONTEIRO Joao LEPENIES Bernd THEPAUT Michel FIESCHI Franck MRÁZKOVÁ Jana WIMMEROVÁ Michaela SCHUHMACHER Frank GOTZE Sebastian GRUNSTEIN Dan GUO Xiaoqiang HAHM Heung Sik KANDASAMY Jeyakumar LEONORI Daniele MARTIN Christopher E. PARAMESWARAPPA Sharavathi G. PASARI Sandip SCHLEGEL Mark K. TANAKA Hidenori XIAO Guozhi YANG You PEREIRA Claney L. ANISH Chakkumkal SEEBERGER Peter H.

Druh Článek v odborném periodiku
Časopis / Zdroj Proceedings of the National Academy of Sciences of the United States of America
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
Doi http://dx.doi.org/10.1073/pnas.1800853116
Klíčová slova glycan arrays; microbial antigens; immune receptors; antiglycan antibodies; bacterial lectins
Popis Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein-glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.
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