Informace o publikaci

Fibroblast growth factor receptor influences primary cilium length through an interaction with intestinal cell kinase

Autoři

BOSÁKOVÁ Michaela NITĂ Alexandru GREGOR Tomáš VAŘECHA Miroslav GUDERNOVÁ Iva FAFÍLEK Bohumil BÁRTA Tomáš BASHEER Neha POOVAKULATHU ABRAHAM Sara BÁLEK Lukáš TOMANOVÁ Markéta FIALOVÁ KUČEROVÁ Jana BOSÁK Juraj POTĚŠIL David ZIEBA Jennifer SONG Jieun KONIK Peter PARK Sohyun DURAN Ivan ZDRÁHAL Zbyněk ŠMAJS David JANSEN Gert FU Zheng KO Hyuk Wan HAMPL Aleš TRANTÍREK Lukáš KRAKOW Deborah KREJČÍ Pavel

Druh Článek v odborném periodiku
Časopis / Zdroj Proceedings of the National Academy of Sciences of the United States of America
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1073/pnas.1800338116
Klíčová slova fibroblast growth factor; FGFR; intestinal cell kinase; ICK; cilia length
Popis Vertebrate primary cilium is a Hedgehog signaling center but the extent of its involvement in other signaling systems is less well understood. This report delineates a mechanism by which fibroblast growth factor (FGF) controls primary cilia. Employing proteomic approaches to characterize proteins associated with the FGF-receptor, FGFR3, we identified the serine/threonine kinase intestinal cell kinase (ICK) as an FGFR interactor. ICK is involved in ciliogenesis and participates in control of ciliary length. FGF signaling partially abolished ICK's kinase activity, through FGFR-mediated ICK phosphorylation at conserved residue Tyr15, which interfered with optimal ATP binding. Activation of the FGF signaling pathway affected both primary cilia length and function in a manner consistent with cilia effects caused by inhibition of ICK activity. Moreover, knockdown and knockout of ICK rescued the FGF-mediated effect on cilia. We provide conclusive evidence that FGF signaling controls cilia via interaction with ICK.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info