Informace o publikaci

Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring

Autoři

PILAT Zdenek JEZEK Jan KIZOVSKY Martin KLEMENTOVA Tereza KRATKY Stanislav SOBOTA Jaroslav SAMEK Ota ZEMANEK Pavel BURYŠKA Tomáš DAMBORSKÝ Jiří PROKOP Zbyněk

Rok publikování 2019
Druh Článek ve sborníku
Konference 2019 PHOTONICS & ELECTROMAGNETICS RESEARCH SYMPOSIUM - SPRING (PIERS-SPRING)
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://ieeexplore.ieee.org/document/9017521
Doi http://dx.doi.org/10.1109/PIERS-Spring46901.2019.9017521
Klíčová slova Raman Spectroscopy; 4-Aminothiophenol; Plasmonics
Popis Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in millimolar and even submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info