Séverine Jansen, PhD.

Curriculum Vitae

Séverine Jansen

• Born in Strasbourg, France, on January 18, 1977

Department

• Protein Structure and Dynamics
  CEITEC MU
  Kamenice 753/5, Brno, 625 00
  Czech republic

Position

• Senior Researcher

Education and Academic Qualifications

• 2007: PhD, Chemical Ecology and Ecotoxicology, Lund University, Sweden.
• 2002: M.S., Molecular and Cellular Biology, Louis Pasteur University, Strasbourg, France.
• 1999: BsC, Chemistry and Biology, Louis Pasteur University, Strasbourg, France.

Employment

• since 2012 : Senior researcher, CEITEC, National Centre for Biomolecular Research, Brno, Czech republic.
• 2009-2012: post doctoral position, National Centre for Biomolecular Research, Brno, Czech republic.

Teaching Activities

• Bsc students: Václav Dvorský, Jan Jurica, Vladimir Jonas, Petra Ježková

Scientific and Research Activities

• My main research is the study of interactions of proteins with their binding partners,and the study of their phosphorylation. Microtubule associated proteins: We recently performed atomic-resolution conformational analysis of MAP2c (consisting of 466 amino acids) and compared the results with homologous protein tau. The analysis revealed presence of multiple functionally important pre-structured motifs, highly correlated with regions of known or proposed functions. This result documents clear evidence of structure-function relationship in intrinsically disordered proteins. We further characterized PKA phosphorylation pattern and kinetics of MAP2c and described its interactions with several protein partners.
• Proteins involved in plant hormone signalling: We explained effects of Mg(2+) binding and pseudo-phosphorylation on structural ensembles and dynamics of receiver domain of CKI1 sensory histidine kinase.
• Insect chemosensory Proteins: we determined the structure of the Chemosensory Protein of Bombyx mori

Internship

• 2004: One year exchange student, Masaryk University, Brno, Czech Republic

Major Publications

• MELKOVÁ, Kateřina, Vojtěch ZAPLETAL, Subhash NARASIMHAN, Séverine JANSEN, Jozef HRITZ, R. SKRABANA, M. ZWECKSTETTER, M. RINGKJOBING JENSEN, M. BLACKLEDGE a Lukáš ŽÍDEK. Structure and Functions of Microtubule Associated Proteins Tau and MAP2c: Similarities and Differences. Biomolecules. Basel, Switzerland: MDPI AG, 2019, roč. 9, č. 3, s. 105-136. ISSN 2218-273X. doi:10.3390/biom9030105. URL info
• MELKOVÁ, Kateřina, Vojtěch ZAPLETAL, Séverine JANSEN, Erik NOMILNER, Milan ZACHRDLA, Jozef HRITZ, Jiří NOVÁČEK, M. ZWECKSTETTER, M.R. JENSEN, M. BLACKLEDGE a Lukáš ŽÍDEK. Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2018, roč. 293, č. 34, s. 13297-13309. ISSN 0021-9258. doi:10.1074/jbc.RA118.001769. URL info
• ŠPAČKOVÁ, Naděžda, Zuzana TROŠANOVÁ, F. SEBESTA, Séverine JANSEN, J.V. BURDA, Pavel SRB, Milan ZACHRDLA, Lukáš ŽÍDEK a Jiří KOZELKA. Protein environment affects the water-tryptophan binding mode. MD, QM/MM, and NMR studies of engrailed homeodomain mutants. Physical Chemistry Chemical Physics. CAMBRIDGE: Royal Society of Chemistry, 2018, roč. 20, č. 18, s. 12664-12677. ISSN 1463-9076. doi:10.1039/c7cp08623g. URL info
• JANSEN, Séverine, Kateřina MELKOVÁ, Zuzana TROŠANOVÁ, Kateřina HANÁKOVÁ, Milan ZACHRDLA, Jiří NOVÁČEK, Erik ŽUPA, Zbyněk ZDRÁHAL, Jozef HRITZ a Lukáš ŽÍDEK. Quantitative mapping of microtubule-associated protein 2c (MAP2c) phosphorylation and regulatory protein 14-3-3 zeta-binding sites reveals key differences between MAP2c and its homolog Tau. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2017, roč. 292, č. 16, s. 6715-6727. ISSN 0021-9258. doi:10.1074/jbc.M116.771097. URL info
• OTRUSINOVÁ, Olga, Gabriel DEMO, Petr PADRTA, Zuzana JASEŇÁKOVÁ, Blanka PEKÁROVÁ, Zuzana GELOVÁ, Agnieszka SZMITKOWSKA, Pavel KADEŘÁVEK, Séverine JANSEN, Milan ZACHRDLA, Tomáš KLUMPLER, Jaromír MAREK, Jozef HRITZ, Lubomír JANDA, Hideo IWAI, Michaela WIMMEROVÁ, Jan HEJÁTKO a Lukáš ŽÍDEK. Conformational dynamics are a key factor in signaling mediated by the receiver domain of a sensor histidine kinase from Arabidopsis thaliana. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2017, roč. 292, č. 42, s. 17525-17540. ISSN 0021-9258. doi:10.1074/jbc.M117.790212. URL info
• VÍCHA, Jan, Martin BABINSKÝ, Gabriel DEMO, Olga OTRUSINOVÁ, Séverine JANSEN, Blanka PEKÁROVÁ, Lukáš ŽÍDEK a Markéta MUNZAROVÁ. The influence of Mg2+ coordination on 13C and 15N chemical shifts in CKI1RD protein domain from experiment and molecular dynamics/density functional theory calculations. Proteins: Structure, Function, and Bioinformatics. 2016, roč. 84, č. 5, s. 686-699. ISSN 0887-3585. doi:10.1002/prot.25019. info
• PEKÁROVÁ, Blanka, Tomáš KLUMPLER, Olga TŘÍSKOVÁ, Jakub HORÁK, Séverine JANSEN, Radka DOPITOVÁ, Petra BORKOVCOVÁ, Veronika PAPOUŠKOVÁ, Eliška NEJEDLÁ, Vladimír SKLENÁŘ, Jaromír MAREK, Lukáš ŽÍDEK, Jan HEJÁTKO a Lubomír JANDA. Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana. The Plant Journal. England: BLACKWELL PUBLISHING, 2011, roč. 67, č. 5, s. 827-839. ISSN 0960-7412. doi:10.1111/j.1365-313X.2011.04637.x. URL info
• JANSEN, Séverine, Josef CHMELÍK, Lukáš ŽÍDEK, Petr PADRTA, Petr NOVÁK, Zbyněk ZDRÁHAL, Jean-François PICIMBON, Christer LÖFSTEDT a Vladimír SKLENÁŘ. Structure of Bombyx mori chemosensory protein 1 in solution. Archives of Insect Biochemistry and Physiology. Wiley, 2007, roč. 66, č. 3, s. 135-145. ISSN 0739-4462. info
• JANSEN, Séverine, Lukáš ŽÍDEK, Christer LÖFSTEDT, Jean-Francois PICIMBON a Vladimír SKLENÁŘ. 1H, 13C, and 15N resonance assignment of Bombyx mori chemosensory protein 1 (BmorCSP1). Journal of Biomolecular NMR. ESCOM Science Publishers B.V., 2006, roč. 36, č. 5, s. 47-47. ISSN 0925-2738. info

2019/06/27