Séverine Jansen, PhD.

Curriculum Vitae

Séverine Jansen

• Born in Strasbourg, France, on January 18, 1977

Department

• Protein Structure and Dynamics
  CEITEC MU
  Kamenice 753/5, Brno, 625 00
  Czech republic

Position

• Senior Researcher

Education and Academic Qualifications

• 2007: PhD, Chemical Ecology and Ecotoxicology, Lund University, Sweden.
• 2002: M.S., Molecular and Cellular Biology, Louis Pasteur University, Strasbourg, France.
• 1999: BsC, Chemistry and Biology, Louis Pasteur University, Strasbourg, France.

Employment

• since 2012 : Senior researcher, CEITEC, National Centre for Biomolecular Research, Brno, Czech republic.
• 2009-2012: post doctoral position, National Centre for Biomolecular Research, Brno, Czech republic.

Teaching Activities

• Bsc students: Václav Dvorský, Jan Jurica, Vladimir Jonas, Petra Ježková

Scientific and Research Activities

• My main research is the study of interactions of proteins with their binding partners,and the study of their phosphorylation. Microtubule associated proteins: We recently performed atomic-resolution conformational analysis of MAP2c (consisting of 466 amino acids) and compared the results with homologous protein tau. The analysis revealed presence of multiple functionally important pre-structured motifs, highly correlated with regions of known or proposed functions. This result documents clear evidence of structure-function relationship in intrinsically disordered proteins. We further characterized PKA phosphorylation pattern and kinetics of MAP2c and described its interactions with several protein partners.
• Proteins involved in plant hormone signalling: We explained effects of Mg(2+) binding and pseudo-phosphorylation on structural ensembles and dynamics of receiver domain of CKI1 sensory histidine kinase.
• Insect chemosensory Proteins: we determined the structure of the Chemosensory Protein of Bombyx mori

Internship

• 2004: One year exchange student, Masaryk University, Brno, Czech Republic

Major Publications

• MELKOVÁ, Kateřina; Vojtěch ZAPLETAL; Subhash NARASIMHAN; Séverine JANSEN; Jozef HRITZ; R. SKRABANA; M. ZWECKSTETTER; M. RINGKJOBING JENSEN; M. BLACKLEDGE a Lukáš ŽÍDEK. Structure and Functions of Microtubule Associated Proteins Tau and MAP2c: Similarities and Differences. Biomolecules. Basel, Switzerland: MDPI AG, 2019, roč. 9, č. 3, s. 105-136. ISSN 2218-273X. Dostupné z: https://doi.org/10.3390/biom9030105. URL info
• MELKOVÁ, Kateřina; Vojtěch ZAPLETAL; Séverine JANSEN; Erik NOMILNER; Milan ZACHRDLA; Jozef HRITZ; Jiří NOVÁČEK; M. ZWECKSTETTER; M.R. JENSEN; M. BLACKLEDGE a Lukáš ŽÍDEK. Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2018, roč. 293, č. 34, s. 13297-13309. ISSN 0021-9258. Dostupné z: https://doi.org/10.1074/jbc.RA118.001769. URL info
• ŠPAČKOVÁ, Naděžda; Zuzana TROŠANOVÁ; F. SEBESTA; Séverine JANSEN; J.V. BURDA; Pavel SRB; Milan ZACHRDLA; Lukáš ŽÍDEK a Jiří KOZELKA. Protein environment affects the water-tryptophan binding mode. MD, QM/MM, and NMR studies of engrailed homeodomain mutants. Physical Chemistry Chemical Physics. CAMBRIDGE: Royal Society of Chemistry, 2018, roč. 20, č. 18, s. 12664-12677. ISSN 1463-9076. Dostupné z: https://doi.org/10.1039/c7cp08623g. URL info
• JANSEN, Séverine; Kateřina MELKOVÁ; Zuzana TROŠANOVÁ; Kateřina HANÁKOVÁ; Milan ZACHRDLA; Jiří NOVÁČEK; Erik ŽUPA; Zbyněk ZDRÁHAL; Jozef HRITZ a Lukáš ŽÍDEK. Quantitative mapping of microtubule-associated protein 2c (MAP2c) phosphorylation and regulatory protein 14-3-3 zeta-binding sites reveals key differences between MAP2c and its homolog Tau. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2017, roč. 292, č. 16, s. 6715-6727. ISSN 0021-9258. Dostupné z: https://doi.org/10.1074/jbc.M116.771097. URL info
• OTRUSINOVÁ, Olga; Gabriel DEMO; Petr PADRTA; Zuzana JASEŇÁKOVÁ; Blanka PEKÁROVÁ; Zuzana GELOVÁ; Agnieszka SZMITKOWSKA; Pavel KADEŘÁVEK; Séverine JANSEN; Milan ZACHRDLA; Tomáš KLUMPLER; Jaromír MAREK; Jozef HRITZ; Lubomír JANDA; Hideo IWAI; Michaela WIMMEROVÁ; Jan HEJÁTKO a Lukáš ŽÍDEK. Conformational dynamics are a key factor in signaling mediated by the receiver domain of a sensor histidine kinase from Arabidopsis thaliana. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2017, roč. 292, č. 42, s. 17525-17540. ISSN 0021-9258. Dostupné z: https://doi.org/10.1074/jbc.M117.790212. URL info
• VÍCHA, Jan; Martin BABINSKÝ; Gabriel DEMO; Olga OTRUSINOVÁ; Séverine JANSEN; Blanka PEKÁROVÁ; Lukáš ŽÍDEK a Markéta MUNZAROVÁ. The influence of Mg2+ coordination on 13C and 15N chemical shifts in CKI1RD protein domain from experiment and molecular dynamics/density functional theory calculations. Proteins: Structure, Function, and Bioinformatics. 2016, roč. 84, č. 5, s. 686-699. ISSN 0887-3585. Dostupné z: https://doi.org/10.1002/prot.25019. info
• PEKÁROVÁ, Blanka; Tomáš KLUMPLER; Olga TŘÍSKOVÁ; Jakub HORÁK; Séverine JANSEN; Radka DOPITOVÁ; Petra BORKOVCOVÁ; Veronika PAPOUŠKOVÁ; Eliška NEJEDLÁ; Vladimír SKLENÁŘ; Jaromír MAREK; Lukáš ŽÍDEK; Jan HEJÁTKO a Lubomír JANDA. Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana. The Plant Journal. England: BLACKWELL PUBLISHING, 2011, roč. 67, č. 5, s. 827-839. ISSN 0960-7412. Dostupné z: https://doi.org/10.1111/j.1365-313X.2011.04637.x. URL info
• JANSEN, Séverine; Josef CHMELÍK; Lukáš ŽÍDEK; Petr PADRTA; Petr NOVÁK; Zbyněk ZDRÁHAL; Jean-François PICIMBON; Christer LÖFSTEDT a Vladimír SKLENÁŘ. Structure of Bombyx mori chemosensory protein 1 in solution. Archives of Insect Biochemistry and Physiology. Wiley, 2007, roč. 66, č. 3, s. 135-145. ISSN 0739-4462. info
• JANSEN, Séverine; Lukáš ŽÍDEK; Christer LÖFSTEDT; Jean-Francois PICIMBON a Vladimír SKLENÁŘ. 1H, 13C, and 15N resonance assignment of Bombyx mori chemosensory protein 1 (BmorCSP1). Journal of Biomolecular NMR. ESCOM Science Publishers B.V., 2006, roč. 36, č. 5, s. 47-47. ISSN 0925-2738. info

2019/06/27