Informace o publikaci
Vinylsulfonamide and Acrylamide Modification of DNA for Cross-linking with Proteins
| Autoři | |
|---|---|
| Rok publikování | 2013 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Angewandte Chemie International Edition |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://onlinelibrary.wiley.com/doi/10.1002/anie.201303577/abstract |
| Doi | http://dx.doi.org/10.1002/anie.201303577 |
| Obor | Biochemie |
| Klíčová slova | bioorthogonal chemistry; DNA; DNA polymerase; Michael additions; proteins |
| Popis | Bioorthogonal covalent cross-linking of DNA-binding proteins (p53) to DNA was achieved through novel DNA probes bearing a reactive vinylsulfonamide (VS) group. The VS-modified dCTP served as building block for polymerase synthesis of modified DNA, which was readily conjugated with cysteine-containing peptides and proteins by Michael addition. |