Informace o publikaci

Aqueous Heck Cross-Coupling Preparation of Acrylate-Modified Nucleotides and Nucleoside Triphosphates for Polymerase Synthesis of Acrylate-Labeled DNA

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DADOVÁ Jitka VIDLÁKOVÁ Pavlína POHL Radek HAVRAN Luděk FOJTA Miroslav HOCEK Michal

Rok publikování 2013
Druh Článek v odborném periodiku
Časopis / Zdroj The journal of organic chemistry
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
Doi http://dx.doi.org/10.1021/jo4011574
Obor Biochemie
Klíčová slova DNA polymerase; DNA labelling; nucleoside triphosphates
Popis Aqueous-phase Heck coupling methodology was developed for direct attachment of butyl acrylate to 5-iodoracil, 5-iodocytosine, 7-iodo-7-deazaadenine, and 7-iodo-7-deazaguanine 2'-deoxyribonucleoside 5'-O-monophosphates (dNMPs) and 5'-O-triphosphates (dNTPs) and compared with the classical approach of phosphorylation of the corresponding modified nucleosides. The acrylate-modified dN(BA)TPs (N = A, C, and U) were good substrates for DNA polymerases and were used for enzymatic synthesis of acrylate-modified DNA by primer extension, whereas dG(BA)TP was an inhibitor of polymerases.

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