Informace o publikaci

Dual effect of ethanol on inward rectifier potassium current Ik1 in rat ventricular myocytes



Rok publikování 2014
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Physiology and Pharmacology
Fakulta / Pracoviště MU

Lékařská fakulta

Obor Fyziologie
Klíčová slova Arrhythmia; Dual effect; Ethanol; Inward rectifier; Rat ventricular action potential model; Rat ventricular myocytes
Popis Alcohol consumption may result in electrocardiographic changes and arrhythmias. Important role of modifications of the inward rectifier potassium current IK1 in arrhythmogenesis is well established. Considering lack of relevant data, we aimed at studying the effect of 0.2-200 mM ethanol on IK1 in enzymatically isolated rat right ventricular myocytes using the whole cell patch-clamp technique at 23±1°C. Ethanol reversibly affected IK1 in a dual way. At a very low concentration of 0.8 mM (~0.004%), ethanol significantly decreased IK1 by 6.9±2.7%. However, at concentrations of ethanol >/- 20 mM (~0.09%), IK1 was conversely significantly increased (by 16.6±4.0% at 20 mM and 24.5±2.4% at 80 mM). The steady-state IK1 increase was regularly preceded by its transient decrease at the beginning of ethanol application. Under 2 and 8 mM ethanol, IK1 was decreased at the steady-state in some cells but increased in others. Both effects were voltage-independent. In agreement with the observed effects of ethanol on IK1, a transient action potential (AP) prolongation followed by its final shortening were observed after the application of ethanol in a low concentration of 8 mM (~0.04%). Under the effect of 0.8 mM ethanol, only AP prolongation was apparent which agreed well with the above described IK1 decrease. Other AP characteristics remained unaltered in both concentrations. These observations corresponded with the results of mathematical simulations in a model of the rat ventricular myocyte. To summarize, changes of the cardiac IK1 under ethanol at concentrations relevant to the current alcohol consumption were first demonstrated in ventricular myocytes in this study. The observed dual ethanol effect suggests at least two underlying mechanisms that remain to be clarified. The ethanol-induced IK1 changes might contribute to the reported alterations of cardiac electrophysiology related to alcohol consumption.
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