IDENTIFICATION OF HUMAN MICROBIOME IN HARD-TO-HEAL WOUNDS BY MALDI-TOF MS AND SEQUENCING

• Autoři: CHUDOBOVA Dagmar; CIHALOVA Kristyna; GURÁŇ Roman; DOSTALOVA Simona; SMERKOVA Kristyna; VESELÝ Radek; GUMULEC Jaromír; HEGER Zbynek; MASAŘÍK Michal; ADAM Vojtěch; KIZEK René
• Rok publikování: 2014
• Druh: Článek ve sborníku
• Konference: CECE 2014: 11th International Interdisciplinary Meeting on Bioanalysis
• Fakulta / Pracoviště MU: Lékařská fakulta
• www: http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf
• Obor: Genetika a molekulární biologie
• Klíčová slova: HARD-TO-HEAL WOUNDS; MALDI-TOF MS; SEQUENCING
• Popis: This study is focused on the isolation, characterization and identification of the most abundant microorganisms in superficial wounds of patients (n = 50) suffering from bacterial infection. Primarily, the identification of the strains was carried out through Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) based on comparison of protein profiles (2-30 kDa) with database. Sanger sequencing of 16S bacterial gene was used for confirmation if the score was < 2.

Související projekty

• CEITEC - středoevropský technologický institut