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Identification of a Novel Retrotransposon with Sex Chromosome-Specific Distribution in Silene latifolia

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KRÁLOVÁ Tereza CEGAN Radim KUBÁT Zdeněk VRÁNA Jan VYSKOT Boris VOGEL Ivan KEJNOVSKÝ Eduard HOBZA Roman

Rok publikování 2014
Druh Článek v odborném periodiku
Časopis / Zdroj Cytogenetic and Genome research
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://www.karger.com/Article/Pdf/362142
Doi http://dx.doi.org/10.1159/000362142
Obor Genetika a molekulární biologie
Klíčová slova Microdissection; Sex chromosomes; Silene latifolia (white campion); Retrotransposons
Popis Silene latifolia is a dioecious plant species with chromosomal sex determination. Although the evolution of sex chromosomes in S. latifolia has been the subject of numerous studies, a global view of X chromosome structure in this species is still missing. Here, we combine X chromosome microdissection and BAC library screening to isolate new X chromosome-linked sequences. Out of 8 identified BAC clones, only BAC 86M14 showed an X-preferential signal after FISH experiments. Further analysis revealed the existence of the Athila retroelement which is enriched in the X chromosome and nearly absent in the Y chromosome. Based on previous data, the Athila retroelement belongs to the CL3 group of most repetitive sequences in the S. latifolia genome. Structural, transcriptomics and phylogenetic analyses revealed that Athila CL3 represents an old clade in the Athila lineage. We propose a mechanism responsible for Athila CL3 distribution in the S. latifolia genome. (C) 2014 S. Karger AG, Basel

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