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Electrochemical and Spectral Behavior of MicroRNA (miR-34a-5p)

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VEČEŘOVÁ Aneta HUDCOVÁ Kristýna PILAŘOVÁ Iveta MASAŘÍK Michal TRNKOVÁ Libuše

Rok publikování 2015
Druh Článek ve sborníku
Konference XXXV. Moderní elektrochemické metody
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Obor Elektrochemie
Klíčová slova miR-34a-5p; human cancer; miDNA; cyclic voltammetry; linear sweep voltammetry; circular dichroic spectroscopy; mercury electrode
Popis MicroRNAs are a family of small noncoding RNAs (21-25 nucleotides), negatively regulating gene expresion at the post-transcriptional level. Their functions are critical to normal cellular processes such as differentiation and apoptosis. recent studies demonstrated that deregulated miRNA expression contributes to the malignant phenotype and for this reason miRNAs play a key role as both diagnostic markers and therapeutic targets for many different tumor types (colorectal cancer, breast cancer, lung cancer, prostate cancer, etc.). Great attention is devoted to the study of miR-34a-5p related to head and neck cancer and also prostate cancer. This miRNA is a direct transcriptional target of p53 and its overexpression after p53 activation mediates some of the key tumor suppressive effects of p53 via induction of growth arrest, apoptisis or senescence. Our contribution is focused on the comparison of the structure of miRNA, based on ribose, and its miDNA analogues, based on deoxyribose and containing U or T in this sequence, by using voltammetric methods (cyclic voltammetry - CV or linear sweep voltammetry - LSV). The voltammetric experiment was completed with CD (circular dichroism) spectra.
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