Informace o publikaci

Characterisation of an unusual telomere motif (TTTTTTAGGG)(n) in the plant Cestrum elegans (Solanaceae), a species with a large genome

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PEŠKA Vratislav FAJKUS Petr FOJTOVÁ Miloslava DVOŘÁČKOVÁ Martina HAPALA Jan DVOŘÁČEK Vojtěch POLANSKÁ Pavla LEITCH Andrew R. SÝKOROVÁ Eva FAJKUS Jiří

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj The Plant Journal
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://onlinelibrary.wiley.com/doi/10.1111/tpj.12839/epdf
Doi http://dx.doi.org/10.1111/tpj.12839
Obor Genetika a molekulární biologie
Klíčová slova Cestrum elegans; NGS analysis; RepeatExplorer; telomerase; telomeric sequence; unusual telomere; GenBank KM573817-573822
Přiložené soubory
Popis The characterization of unusual telomere sequence sheds light on patterns of telomere evolution, maintenance and function. Plant species from the closely related genera Cestrum, Vestia and Sessea (family Solanaceae) lack known plant telomeric sequences. Here we characterize the telomere of Cestrum elegans, work that was a challenge because of its large genome size and few chromosomes (1C 9.76pg; n=8). Significance Statement We previously found that plants from related genera Cestrum, Vestia and Sessea (Solanaceae) lack known telomeric sequences. Here we characterise the Cestrum elegans telomere and the mechanism of its synthesis. We developed an approach combining NGS of untreated and terminally-truncated genomic DNA. We identified, using the RepeatExplorer bioinformatic pipeline, an unusual telomere repeat (TTTTTTAGGG)(n) synthesized by telomerase. That finding will enable studies on evolutionary divergence of telomeres and telomerase, shedding light on telomere interactions and functions.
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