Informace o publikaci

Quantitative Profiling of Immune Repertoires for Minor Lymphocyte Counts Using Unique Molecular Identifiers

Autoři	EGOROV Evgeny S. MERZLYAK Ekaterina M. SHELENKOV Andrew A. BRITANOVA Olga V. SHARONOV George V. STAROVEROV Dmitriy B. BOLOTIN Dmitriy A. DAVYDOV Alexey Nikolayevich BARSOVA Ekaterina LEBEDEV Yuriy B. SHUGAY Mikhail CHUDAKOV Dmitriy
Rok publikování	2015
Druh	Článek v odborném periodiku
Časopis / Zdroj	Journal of immunology
Fakulta / Pracoviště MU	Středoevropský technologický institut
Citace
www	http://www.jimmunol.org/content/194/12/6155
Doi	http://dx.doi.org/10.4049/jimmunol.1500215
Obor	Epidemiologie, infekční nemoci a klinická imunologie
Klíčová slova	T-CELL-RECEPTOR; ANTIBODY REPERTOIRE; DEEP; SEQUENCE; PCR; RECOMBINATION; LIBRARIES; RESPONSES; SELECTION; PLATFORM
Popis	Emerging high-throughput sequencing methods for the analyses of complex structure of TCR and BCR repertoires give a powerful impulse to adaptive immunity studies. However, there are still essential technical obstacles for performing a truly quantitative analysis. Specifically, it remains challenging to obtain comprehensive information on the clonal composition of small lymphocyte populations, such as Ag-specific, functional, or tissue-resident cell subsets isolated by sorting, microdissection, or fine needle aspirates. In this study, we report a robust approach based on unique molecular identifiers that allows profiling Ag receptors for several hundred to thousand lymphocytes while preserving qualitative and quantitative information on clonal composition of the sample. We also describe several general features regarding the data analysis with unique molecular identifiers that are critical for accurate counting of starting molecules in high-throughput sequencing applications.